Custom Peptide Synthesis Research Papers

Custom Peptide Synthesis Research Papers

Methods Mol Biol. 2011;692:265-74.

Custom synthesis of autoinducers and their analogues.

Igarashi J, Suga H.

Abstract

Bacterial quorum sensing (QS) system is a unique target for the development of a new class of drugs that potentially control pathogenicity and attenuate virulence. Thus, it has been of significant interest to discover small organic molecules that modulate QS circuits by competing with the signaling molecules, or so-called autoinducers (AIs), for binding to QS proteins. In this chapter, we summarize synthetic methodology for custom QS agonists and antagonists against the Lux system in Gram-negative bacteria.

J Immunoassay. 1980;1(1):129-47.

"Custom" synthesis of radioligands for RIA through activated esters. I. Testosterone.

Tantchou JK, Slaunwhite WR Jr.

Abstract

The current method labeling small molecules for radioimmunoassay by coupling iodohistamine to haptens through a mixed anhydride reaction is unacceptable to clinical laboratories. Therefore, we propose the use of a simple two-step procedure: treatment of 125I-2-iodohistamine with the activated ester of a small molecule followed by thin layer chromatography to remove unlabeled ligand. Only one radioactive substance, 125I-2-iodohistamine, need be stocked, and the availability of labeled ligands is limited only by the number of nonradioactive activated esters. This principle is illustrated by the use of testosterone. N-Hydroxysuccinimidyl esters of testosterone hemisuccinate and of testosterone-3-carboxymethyloxime were coupled to 2-iodohistamine, 125I-2-iodohistamine or to 125I-2,5-diiodohistamine. Optimum conditions required reaction of 20-50 fold molar excess of ester in 75 microL of tetrahydrofuran with iodohistamine in 75 microL of buffer at pH 8.5 for 30 min at 4 degrees. The reaction mixture was applied directly to a pre-absorbent TLC plate coated with silica gel and run in the system, benzene:ethanol:acetic acid, 75:24:1 (v:v:v). The desired radioligand was eluted in 85% yield.

Custom synthesis of molecular imprinted polymers for biotechnological application: Preparation of a polymer selective for tylosin

Purchase

Sergey Piletsky^{, , a}, Elena Piletska^a, Kal Karim^a, Graham Foster^b, Colton Legge^b and Anthony Turner^a

^a Institute of BioScience and Technology, Cranfield University, Silsoe, Bedfordshire MK45 4DT, UK

^b GlaxoSmithKline Research and Development, Gunnels Wood Road, Stevenage, Herts SG1 2NY, UK

Received 6 January 2003;

revised 12 June 2003;

accepted 24 June 2003. ;

Available online 26 August 2003. http://www.sciencedirect.com/science/article/pii/S0003267003008146

Abstract

A molecularly imprinted polymer (MIP) selective for tylosin was designed and synthesised using a computational method (MIP “dialling”). In re-binding experiments the MIP demonstrated high affinity for tylosin in aqueous solutions and in organic solvents. The synthesised polymer was tested for re-binding with the template and related metabolites such as tylactone, narbomycin and picromycin. The HPLC analysis showed that the computationally designed polymer is specific and capable of separating the template from its structural analogues. The MIP was capable of recovering tylosin from broth samples. The polymer capacity for tylosin was estimated as 6.4 mg/g for MIP, which was suitable for practical application and tylosin recovery from broth samples. Among the advantages of this was the possibility to adsorb tylosin from a complex media with easy removal of oils and other impurities which are present in significant quantities, which can create problems for its chromatographic purification procedure. The MIP “dialling” procedure can have a general significance for the fast preparation of specific adsorbents for biotechnological applications.

Author Keywords: Tylosin; Computational design; Molecularly imprinted polymer

Article Outline

1. Introduction

2. Materials and methods

2.1. Reagents

2.2. Molecular modelling

2.3. Preparation of molecularly imprinted polymers

2.4. Solid-phase extraction (SPE)

2.5. HPLC analysis

3. Results and discussion

3.1. MIP “dialling” protocol

3.2. Polymer design

3.3. SPE analysis

3.4. HPLC experiments

4. Conclusions

Acknowledgements

References

Nature Biotechnology 18, 345 - 348 (2000)
doi:10.1038/73815

Custom fluorescent-nucleotide synthesis as an alternative method for nucleic acid labeling

Octavian Henegariu¹, Patricia Bray-Ward¹ & David C. Ward¹

Abstracthttp://www.nature.com/nbt/journal/v18/n3/abs/nbt0300_345.html

The variety of potentially useful dyes or haptenes available for fluorescent nucleic acid hybridization assays is far greater than what can be obtained from commercial sources ^{1, 2}. Since this diversity could be useful in many laboratory applications, we have developed a simple and inexpensive procedure for preparing nonpurified labeled nucleotides ^{3, 4, 5}, for use in common nucleic acid labeling reactions, such as PCR and nick translation. The modified nucleotides were synthesized by coupling allylamine-dUTP to the succinimidyl-ester derivatives of the fluorescent dyes or haptenes such as biotin or digoxigenin, which require fluorescently labeled proteins for detection. This method allows custom preparation of most common fluorescent nucleotides and rapid testing of new ones, while reducing the cost of procedures such as multiplex fluorescent in situ hybridization (M-FISH) by 100-200 fold.

topof page

1. Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510

Correspondence to: Octavian Henegariu¹ e-mail: ohenegar@yahoo.com.

Titre du document / Document title http://cat.inist.fr/?aModele=afficheN&cpsidt=14553008

Custom synthesis for drug discovery

Auteur(s) / Author(s)

ROUHI A. Maureen ;

Revue / Journal Title

Chemical & engineering news ISSN 0009-2347

Source / Source

2003, vol. 81, n^o7, pp. 75-78 [4 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

American Chemical Society, Washington, DC, ETATS-UNIS (1942) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 5061, 35400010415058.0030

Custom synthesis and process development.

Tyson, R.

Chemistry and Industry , pp. 118-22. 15 Feb. 1988

Descriptors: ANTE | Contractors | Manufactures | Drugs

http://md1.csa.com/partners/viewrecord.php?requester=gs&collection=TRD&recid=8819417AN&q=allintitle%3A+custom+synthesis&uid=791001519&setcookie=yes

Conformational study of a custom antibacterial peptide cecropin B1: implications of the lytic activity

S Srisailam - Biochimica et Biophysica Acta (BBA)-Protein Structure …, 2000 - Elsevier
Cecropin B1 (CB1) with two amphipathic α-helical segments is a derivative of the natural antibacterial
peptide, cecropin B. The assays of cell lysis show that, compared with cecropin A (CA), CB1
has a similar ability to lyse bacteria with a higher potency (two- to six-fold higher) in killing ...

Kinetics of membrane lysis by custom lytic peptides and peptide orientations in membrane

HM Chen, AHA Clayton, W Wang… - European Journal of …, 2001 - Wiley Online Library
To aid the development of custom peptide antibiotics, a kinetic study of membrane lysis by cecropin
B (CB) and its analogs, cecropin B1 (CB1) and cecropin B3 (CB3) was carried out to determine
the mechanism by which these peptides disrupt the bilayer structure of liposomes of ...

Crumpled structure of the custom hydrophobic lytic peptide cecropin B3

S Srisailam, TKS Kumar… - European Journal …, 2001 - Wiley Online Library
The solution structure of a custom lytic peptide, cecropin B3 (CB3), having two identical hydrophobic
segments on both the N- and C-termini, was investigated by two-dimensional NMR
spectroscopy. The need to determine the structure of this peptide is rooted in its specific ...

[44] Custom-designed synthetic peptide immunoassays for distinguishing HIV type 1 and type 2 infections

JW Gnann Jr, LL Smith… - Methods in enzymology, 1989 - Elsevier
... Elsevier Inc. Permissions & Reprints. [44] Custom-designed synthetic peptide
immunoassays for distinguishing HIV type 1 and type 2 infections. This article is not
included in your organization's subscription. However, you may ...

Structure and function of a custom anticancer peptide, CB1a

JM Wu, PS Jan, HC Yu, HY Haung, HJ Fang… - Peptides, 2009 - Elsevier
Several natural antimicrobial peptides including cecropins, magainins and melittins have been
found to kill cancer cells. However, their efficacy may not be adequate for their development
as anticancer agents. In this study, we used a natural antimicrobial peptide, cecropin B ...

[PDF] Custom Peptide Mixes for Cytokine Flow Cytometry

[PDF] from typepad.comK Willmann, JA Zawadzki… - maeckerlab.typepad.com
Overlapping peptide mixtures have several benefits over the use of whole protein and whole
viral lysate prepara- tions. First, because they are synthetically derived, they can be made
reproducibly, with less potential lot-to-lot variability. Second, whole protein antigens ...

CUSTOM PEPTIDE MANUFACTURING: COMPANIES MARKET PRODUCTS ON PURITY AND PRICE ISSUES

K LISZEWSKI - Genetic engineering news, 1998 - cat.inist.fr
... Titre du document / Document title. CUSTOM PEPTIDE MANUFACTURING : COMPANIES
MARKET PRODUCTS ON PURITY AND PRICE ISSUES. Auteur(s) / Author(s). LISZEWSKI K. ;
Revue / Journal Title. Genetic engineering news ISSN 0270-6377 Source / Source. 1998, vol ...

[PDF] Use of JMP® for Optimizing a Custom Peptide Microarray to Identify Autoantibodies for Multiple Sclerosis

[PDF] from sas.comA Rahman, W Robinson, H Garren, R King… - sas.com
Summary ...................................................................................................... 1 Introduction ....................
........................................................................... ... ... USe Of JMP® fOr OPtIMIzIng A CUStOM PePtIde
MICrOArrAy ... Design of experiment (DOE) was utilized to optimize a peptide microarray ...

Custom Peptide Synthesis

SP Kits - sciencemag.org
Science/AAAS. AAAS.ORG; Feedback; Help; Librarians. Science Magazine
Advanced. Google Indexer; Alerts; Access Rights; My Account; ...

Custom microarray analysis of exercise-regulated genes identifies a peptide-precursor with antidepressant-like effects

JG Hunsberger - 2007 - gradworks.umi.com
Abstract: Depression is a serious mental illness that impacts many people each year. Current
treatments which focus on monoaminergic break down and reuptake take weeks to months before
a therapeutic effect is achieved. Therefore, there is great need for the development of new ...